second antibody造句

Methods : sandwich elisa assay was used , w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody . the second antibody - hrp conjugate was added for coloration . standard curve was obtained by shla - i standard reagent in serial dilution . the amount of shla - i in the samples was determined : 1
方法：以w6 32包被酶标板，捕捉样品中可溶性hla ，加入一抗2m抗体，再加酶标二抗及底物显色。根据可溶性hla -的不同浓度标准品显色后的od值绘制标准曲线： 1
Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related . through three cycles of screening , the inserted cdna fragments of the positive clones were amplified by pcr and sequenced . the results showed that the inserted cdna fragment from one clone was 1200 bp in length , with a orf of 507 bp which encoded 169 amino acids
Sj43b pgex 6p 1重组质粒的诱导表达、表达产物的乡寸和免疫学性质鉴定分析为获得可溶性的rsj43b月6gsta虫合蛋白，对不同iptg诱导剂浓度、诱导表达温度和诱导表达时间等因素对融合蛋白可溶性表达的影响进行了观察。
It's difficult to find second antibody in a sentence. 用second antibody造句挺难的